Journal: Chinese Herbal Medicines

Article Title: Isoquinoline alkaloids in Coptis chinensis to treat Alzheimer’s disease through promoting growth of Bifidobacterium breve inhibiting abnormal autophagy using a novel AI high-content intelligent imaging system

doi: 10.1016/j.chmed.2026.02.003

Figure Lengend Snippet: Co-administration of C. chinensis and B. breve S1 improved the pathological manifestations of AD mice. (A) Construction of B. breve S1 AD mice (* P < 0.05 ** P < 0.01 *** P < 0.001 vs D1, # P < 0.05 ## P < 0.01 ### P < 0.001 vs D7). (B) Statistical chart of latency time of Barnes maze test in AD mice ( n = 5). (C) Statistical chart of target quadrant ratio of Barnes maze test in mice ( n = 5). (D) Statistical chart of total displacement of Barnes maze test in mice ( n = 5). (E) The content of A β in serum ( n = 5). (F) The content of A β in brain ( n = 5). n. s., no significant difference, # P < 0.05 ### P < 0.001 vs Control group, * P < 0.05 ** P < 0.01 *** P < 0.001 vs Model-free group, n.s. not significant. (G) Results of Congo red staining (arrow: amyloid protein deposition, n = 5, scale bar: 500 μm, × 40). (H) Results of HE staining ( n = 5, scale bar: 50 μm, × 400). (I) Results of TUNEL staining (arrow: apoptotic cells, n = 5, scale bar: 50 μm, × 400).

Article Snippet: TUNEL staining kit (lot number: 01981015) was purchased from Shanghai Epizyme Biomedical Technology Co., Ltd. (Shanghai, China).

Techniques: Control, Staining, TUNEL Assay